Содержание
- 2. BIND: repressors α- turn - α
- 3. DNA & RNA BINDING Zn- fingers Leu-zipper
- 4. -BINDING-INDUCED DEFORMATION MAKES REPRESSOR ACTIVE, and IT BINDS TO DNA BIND ? ? RELEASE: REPRESSOR
- 5. Immunoglobulin
- 6. Standard positions of active sites in protein folds
- 7. There are some with catalytic (Ser-protease) site
- 8. Preferential binding of TS: RIGID enzyme Catalysis: stabilization of the transition state (TS) Theory: Pauling &
- 9. Catalysis: stabilization of the transition state (TS) Theory: Pauling & Holden Experimental verification: Fersht ______ __________
- 10. Catalysis: stabilization of the transition state (TS) Theory: Pauling & Holden Experimental verification: Fersht ______ __________
- 11. Catalytic antibodies ABZYM = AntyBody enZYM Antibodies are selected to TS-like molecule Transition state (TS ‡)
- 12. BIND ? TRANSFORM ? RELEASE: ENZYME Note: small active site chymotrypsin
- 13. Sometimes: Different folds with the same active site: the same biochemical function
- 14. POST-TRANSLATIONAL MODIFICATION Sometimes, only the CHAIN CUT-INDUCED DEFORMATION MAKES THE ENZYME ACTIVE READY Chymotripsinogen active cat.
- 15. Chymotrypsin catalyses hydrolysis of a peptide Spontaneous hydrolysis: very slow
- 16. SER-protease: catalysis
- 17. CHYMOTRYPSIN ACTIVE SITE with INHIBITOR
- 18. Preferential binding of TS: RIGID enzyme F = k1x1 = - k2x2 Ei = (ki /2)(xi)2
- 19. PROTEIN STRUCTURE AT ACTION: BIND ? TRANSFORM ? RELEASE RIGID CATALITIC SITE INDEPENDENT ON OVERALL CHAIN
- 20. Induced fit model for enzyme catalysis. Daniel Edward Koshland, Jr. (1920 – 2007) Hermann Emil Louis
- 21. MOTIONS
- 22. Double sieve: movement of substrate from one active site to another ⇑ tRNAIle Fersht A.R., Dingwall
- 23. Movement in two-domain enzyme: One conformation for binding (and release), another for catalysis Induced fit
- 24. Two-domain dehydrogenases: Universal NAD-binding domain; Individual substrate-binding domain
- 25. Movement in quaternary structure: Hemoglobin vs. myoglobin non-covalent bonding of O2 move of O2 to and
- 26. Kinesin : Linear cyclic motor the simplest one-direction walking machine with cyclic ligand-induced conformational changes and
- 27. Kinesin : Linear cyclic motor the simplest one-direction walking machine with cyclic ligand-induced conformational changes and
- 28. Sir Andrew Fielding Huxley (1917 – 2012) Nobel Prize 1963 Myosin "cross-bridges"
- 29. Механохимический цикл Миозин Актин АТФ → АДФ + Ф 15 ккал/моль в клеточных условиях
- 30. Mechanochemical cycle Myosin Actin
- 31. structure from the X-ray data: Junge, Sielaff, Engelbrecht, Nature, 459, 364 (2009) Rotary motor F0F1-ATP synthase
- 32. Engelbrecht & Junge, FEBS Lett. 414, 485 (1997) Elston, Wang, Oster, Nature, 391, 510 (1998) F0-machine:
- 33. Rotary motor F0F1-ATP synthase ⎯ working cycle of the H+-turbine
- 34. H3O+ binding in Bacillus pseudofirmus Ion binding to the rotor ring of F0F1-ATP synthase H+ binding
- 35. SUMMARY of the course
- 36. PROTEIN PHYSICS Interactions Structures Selection States & transitions
- 37. Intermediates & nuclei Structure prediction & bioinformatics Protein engineering & design Functioning
- 38. Благодарю за внимание … товарищи офизевшие биологи!
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