Содержание
- 2. NATURE OF ANTIGEN-ANTIBODY REACTIONS Lock and Key Concept The combining site of an antibody is located
- 3. AFFINITY AND AVIDITY Affinity Antibody affinity is the strength of the reaction between a single antigenic
- 5. Avidity Avidity is a measure of the overall strength of binding of an antigen with many
- 7. Possible effects on soluble protein of immobilization Protein is shown as having three antigenic sites (epitopes).
- 8. SPECIFICITY AND CROSS REACTIVITY Specificity Specificity refers to the ability of an individual antibody combining site
- 9. Diagnosis of infectious and parasitic diseases and the establishment of detection antibody titers (serodiagnosis); Diagnosis of
- 10. Cross reactivity Cross reactivity refers to the ability of an individual antibody combining site to react
- 11. Agglutination test Agglutination test (agglutinacio - склеивание) - gluing and precipitation of the bacteria under the
- 12. STATEMENT OF MICROAGGLUTINATION TEST
- 13. THE RESULTS OF MICROAGGLUTINATION TEST
- 15. In positive cases precipitate has the form of a thin film of the red blood cells
- 16. PRECIPITATION TEST Principle: When interacting of soluble antigen with antibody in the presence of electrolyte (NaCl)
- 17. THE PRINCIPLE OF PRECIPITATION TEST
- 18. Ring-precipitation test Formation of the Ag-Ab complex - + The test is carried out by layering
- 19. THE PRINCIPLE OF RADIAL IMMUNODIFFUSION TEST The test is carried out in agar gel plates or
- 20. Radial Immunodiffusion (RID)
- 21. Complement fixation test
- 22. Materials and Reagents 1. Sheep erythrocytes suspension (5% suspension of washed sheep RBCs) 2. Hemolysin (rabbit
- 23. The principle of the complement fixation test is illustrated in Figure. Antigen is mixed with the
- 24. Procedure of Complement Fixation Test Complement Fixation Test (CFT) consists of two stage: First step (Complement
- 25. Enzyme linked immunosorbent assay ELISA - it is serological test in which for the visualization of
- 26. IgG IgM Серологические реакции, основанные на использовании меток МЕТКИ Ферментные Флюоресцирующие радиоизотопные
- 29. 96-луночный планшет для ИФА
- 31. Спектрофотометр для ИФА
- 32. ПРИНЦИП НЕПРЯМОГО ИФА
- 34. This variant of ELISA is extremely common for the determination of antigens possessing more than one
- 35. Принцип сэндвич-ИФА
- 37. Конкурентный ИФА для определения антигена (антибиотиков) Конъюгат АБ-носитель Субстрат Антибиотик Специфические АТ Антивидовой конъюгат
- 39. Конкурентный ИФА для определения специфических антител
- 40. ИСПОЛЬЗОВАНИЕ АВИДИН-БИОТИНОВОЙ СИСТЕМЫ В ИФА
- 42. Dot-ИФА
- 43. Сравнительная характеристика dot-ИФА и ИФА.
- 44. ПРИНЦИП ИММУНОХРОМАТОГРАФИЧЕСКОГО АНАЛИЗА
- 45. Оборудование для производства иммунохроматографической тест - системы Презиционный диспенсер автоматический Автоматический гильотинный резак Вакуумный сушильный шкаф
- 46. Иммунохроматографическое определение антигена (I – аналитическая зона, II – контрольная зона)
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