Содержание
- 2. Overview: Life at the Edge The plasma membrane is the boundary that separates the living cell
- 3. Fig. 7-1
- 4. Concept 7.1: Cellular membranes are fluid mosaics of lipids and proteins Phospholipids are the most abundant
- 5. Membrane Models: Scientific Inquiry Membranes have been chemically analyzed and found to be made of proteins
- 6. Fig. 7-2 Hydrophilic head WATER Hydrophobic tail WATER
- 7. In 1935, Hugh Davson and James Danielli proposed a sandwich model in which the phospholipid bilayer
- 8. Fig. 7-3 Phospholipid bilayer Hydrophobic regions of protein Hydrophilic regions of protein
- 9. Freeze-fracture studies of the plasma membrane supported the fluid mosaic model Freeze-fracture is a specialized preparation
- 10. Fig. 7-4 TECHNIQUE Extracellular layer Knife Proteins Inside of extracellular layer RESULTS Inside of cytoplasmic layer
- 11. The Fluidity of Membranes Phospholipids in the plasma membrane can move within the bilayer Most of
- 12. Fig. 7-5 Lateral movement (~107 times per second) Flip-flop (~ once per month) (a) Movement of
- 13. Fig. 7-5a (a) Movement of phospholipids Lateral movement (~107 times per second) Flip-flop (~ once per
- 14. Fig. 7-6 RESULTS Membrane proteins Mouse cell Human cell Hybrid cell Mixed proteins after 1 hour
- 15. As temperatures cool, membranes switch from a fluid state to a solid state The temperature at
- 16. Fig. 7-5b (b) Membrane fluidity Fluid Unsaturated hydrocarbon tails with kinks Viscous Saturated hydro- carbon tails
- 17. The steroid cholesterol has different effects on membrane fluidity at different temperatures At warm temperatures (such
- 18. Fig. 7-5c Cholesterol (c) Cholesterol within the animal cell membrane
- 19. Membrane Proteins and Their Functions A membrane is a collage of different proteins embedded in the
- 20. Fig. 7-7 Fibers of extracellular matrix (ECM) Glyco- protein Microfilaments of cytoskeleton Cholesterol Peripheral proteins Integral
- 21. Peripheral proteins are bound to the surface of the membrane Integral proteins penetrate the hydrophobic core
- 22. Fig. 7-8 N-terminus C-terminus α Helix CYTOPLASMIC SIDE EXTRACELLULAR SIDE
- 23. Six major functions of membrane proteins: Transport Enzymatic activity Signal transduction Cell-cell recognition Intercellular joining Attachment
- 24. Fig. 7-9 (a) Transport ATP (b) Enzymatic activity Enzymes (c) Signal transduction Signal transduction Signaling molecule
- 25. Fig. 7-9ac (a) Transport (b) Enzymatic activity (c) Signal transduction ATP Enzymes Signal transduction Signaling molecule
- 26. Fig. 7-9df (d) Cell-cell recognition Glyco- protein (e) Intercellular joining (f) Attachment to the cytoskeleton and
- 27. The Role of Membrane Carbohydrates in Cell-Cell Recognition Cells recognize each other by binding to surface
- 28. Synthesis and Sidedness of Membranes Membranes have distinct inside and outside faces The asymmetrical distribution of
- 29. Fig. 7-10 ER 1 Transmembrane glycoproteins Secretory protein Glycolipid 2 Golgi apparatus Vesicle 3 4 Secreted
- 30. Concept 7.2: Membrane structure results in selective permeability A cell must exchange materials with its surroundings,
- 31. The Permeability of the Lipid Bilayer Hydrophobic (nonpolar) molecules, such as hydrocarbons, can dissolve in the
- 32. Transport Proteins Transport proteins allow passage of hydrophilic substances across the membrane Some transport proteins, called
- 33. Other transport proteins, called carrier proteins, bind to molecules and change shape to shuttle them across
- 34. Concept 7.3: Passive transport is diffusion of a substance across a membrane with no energy investment
- 35. Fig. 7-11 Molecules of dye Membrane (cross section) WATER Net diffusion Net diffusion Equilibrium (a) Diffusion
- 36. Molecules of dye Fig. 7-11a Membrane (cross section) WATER Net diffusion Net diffusion (a) Diffusion of
- 37. Substances diffuse down their concentration gradient, the difference in concentration of a substance from one area
- 38. (b) Diffusion of two solutes Fig. 7-11b Net diffusion Net diffusion Net diffusion Net diffusion Equilibrium
- 39. Effects of Osmosis on Water Balance Osmosis is the diffusion of water across a selectively permeable
- 40. Lower concentration of solute (sugar) Fig. 7-12 H2O Higher concentration of sugar Selectively permeable membrane Same
- 41. Water Balance of Cells Without Walls Tonicity is the ability of a solution to cause a
- 42. Fig. 7-13 Hypotonic solution (a) Animal cell (b) Plant cell H2O Lysed H2O Turgid (normal) H2O
- 43. Hypertonic or hypotonic environments create osmotic problems for organisms Osmoregulation, the control of water balance, is
- 44. Fig. 7-14 Filling vacuole 50 µm (a) A contractile vacuole fills with fluid that enters from
- 45. Water Balance of Cells with Walls Cell walls help maintain water balance A plant cell in
- 46. Video: Plasmolysis Video: Turgid Elodea Animation: Osmosis In a hypertonic environment, plant cells lose water; eventually,
- 47. Facilitated Diffusion: Passive Transport Aided by Proteins In facilitated diffusion, transport proteins speed the passive movement
- 48. Fig. 7-15 EXTRACELLULAR FLUID Channel protein (a) A channel protein Solute CYTOPLASM Solute Carrier protein (b)
- 49. Carrier proteins undergo a subtle change in shape that translocates the solute-binding site across the membrane
- 50. Some diseases are caused by malfunctions in specific transport systems, for example the kidney disease cystinuria
- 51. Concept 7.4: Active transport uses energy to move solutes against their gradients Facilitated diffusion is still
- 52. The Need for Energy in Active Transport Active transport moves substances against their concentration gradient Active
- 53. Active transport allows cells to maintain concentration gradients that differ from their surroundings The sodium-potassium pump
- 54. Fig. 7-16-1 EXTRACELLULAR FLUID [Na+] high [K+] low Na+ Na+ Na+ [Na+] low [K+] high CYTOPLASM
- 55. Na+ binding stimulates phosphorylation by ATP. Fig. 7-16-2 Na+ Na+ Na+ ATP P ADP 2
- 56. Fig. 7-16-3 Phosphorylation causes the protein to change its shape. Na+ is expelled to the outside.
- 57. Fig. 7-16-4 K+ binds on the extracellular side and triggers release of the phosphate group. P
- 58. Fig. 7-16-5 Loss of the phosphate restores the protein’s original shape. K+ K+ 5
- 59. Fig. 7-16-6 K+ is released, and the cycle repeats. K+ K+ 6
- 60. 2 EXTRACELLULAR FLUID [Na+] high [K+] low [Na+] low [K+] high Na+ Na+ Na+ Na+ Na+
- 61. Fig. 7-17 Passive transport Diffusion Facilitated diffusion Active transport ATP
- 62. How Ion Pumps Maintain Membrane Potential Membrane potential is the voltage difference across a membrane Voltage
- 63. Two combined forces, collectively called the electrochemical gradient, drive the diffusion of ions across a membrane:
- 64. An electrogenic pump is a transport protein that generates voltage across a membrane The sodium-potassium pump
- 65. Fig. 7-18 EXTRACELLULAR FLUID H+ H+ H+ H+ Proton pump + + + H+ H+ +
- 66. Cotransport: Coupled Transport by a Membrane Protein Cotransport occurs when active transport of a solute indirectly
- 67. Fig. 7-19 Proton pump – – – – – – + + + + + +
- 68. Concept 7.5: Bulk transport across the plasma membrane occurs by exocytosis and endocytosis Small molecules and
- 69. Exocytosis In exocytosis, transport vesicles migrate to the membrane, fuse with it, and release their contents
- 70. Endocytosis In endocytosis, the cell takes in macromolecules by forming vesicles from the plasma membrane Endocytosis
- 71. In phagocytosis a cell engulfs a particle in a vacuole The vacuole fuses with a lysosome
- 72. Fig. 7-20 PHAGOCYTOSIS EXTRACELLULAR FLUID CYTOPLASM Pseudopodium “Food”or other particle Food vacuole PINOCYTOSIS 1 µm Pseudopodium
- 73. Fig. 7-20a PHAGOCYTOSIS CYTOPLASM EXTRACELLULAR FLUID Pseudopodium “Food” or other particle Food vacuole Food vacuole Bacterium
- 74. In pinocytosis, molecules are taken up when extracellular fluid is “gulped” into tiny vesicles Animation: Pinocytosis
- 75. Fig. 7-20b PINOCYTOSIS Plasma membrane Vesicle 0.5 µm Pinocytosis vesicles forming (arrows) in a cell lining
- 76. In receptor-mediated endocytosis, binding of ligands to receptors triggers vesicle formation A ligand is any molecule
- 77. Fig. 7-20c RECEPTOR-MEDIATED ENDOCYTOSIS Receptor Coat protein Coated pit Ligand Coat protein Plasma membrane 0.25 µm
- 78. Fig. 7-UN1 Passive transport: Facilitated diffusion Channel protein Carrier protein
- 79. Fig. 7-UN2 Active transport: ATP
- 80. Fig. 7-UN3 Environment: 0.01 M sucrose 0.01 M glucose 0.01 M fructose “Cell” 0.03 M sucrose
- 81. Fig. 7-UN4
- 82. You should now be able to: Define the following terms: amphipathic molecules, aquaporins, diffusion Explain how
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